ACANTHAMOEBA CULBERTSONI PDF

Several species of Acanthamoeba, including A. culbertsoni, A. polyphaga, A. castellanii, A. astronyxis, A. hatchetti, A. rhysodes, A. divionensis. In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyzed Acanthamoeba culbertsoni attachment. They have been implicated in human infections and disease, including Acanthamoeba culbertsoni, Acanthamoeba polyphaga, Acanthamoeba castellanii.

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Acanthamoeba invasion of the central nervous system. Soluble factors present in A.

Author information Article notes Copyright and License information Disclaimer. D Densitometric comparison of lane A1 for A. Exposure of BV-2 cells to A. Proteolytic enzymes of pathogenic and non-pathogenic strains of Acanthamoeba spp. This protein is a homotrimeric type II membrane protein which has a C-terminal region outside the cell and an extracellular region that has homology to the culberrsoni region of members of the TNF family.

Collectively, the results suggest that apoptosis induced by soluble factors in A. In vitromicroglia have been shown to produce an array of chemokines and cytokines in response to Acanthamoeba 31 Acanthamoeba Acanthamoeba keratitis Cutaneous acanthamoebiasis Granulomatous amoebic encephalitis Acanthamoeba infection Culbertsonl mandrillaris Balamuthia amoebic encephalitis Balamuthia infection.

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Pathogen & Environment

Variosea paraphyletic Holomastigida Phalansteriida Artodiscida Varipodida. All wells were read at nm with a correction wavelength of nm. Entamoeba histolytica Amoebiasis Amoebic dysentery Amoebic liver abscess Cutaneous amoebiasis Amoebic brain abscess Amebiasis cutis Entamoeba gingivalis. In the present study, a BV-2 cell in vitro model was used to gain insight into the microglial cell- Acanthamoeba interaction.

Collectively, these results suggest a mode through which A. Retrieved 31 May Thus, although a large-scale screening of chemokine and cytokine mRNA species was not performed, these observations acanthaomeba consistent with the conclusions that i A.

Exp Parasitol ;30 1: On the other hand, treatment with 5 mM 1,phenathroline resulted in abrogation of activity associated with the, and kDa bands. Echinococcus multilocularis metacestode metabolites contain a cysteine protease that digests eotaxin, a CC pro-inflammatory chemokine.

Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity

The blastogenic responses of splenocytes treated with concanavalin A were declined significantly in the experimental group as compared with the control group, but the blastogenic responses of splenocytes treated with polyinosinic acid were not different from the control group. BV-2 cells were maintained in Neurobasal-A medium for 18 h as a negative control or in Neurobasal-A medium supplemented with 0.

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For supernatants of BV-2 cells incubated 8 h with A. Parasite Immunol ;1 4: In summary, it is likely that A. Immunization with culture supernatant in experimental amoebic meningoencephalitis.

In vitro destruction of nerve cell cultures by Acanthamoeba spp.: Role of carbohydrate-mediated adherence in cytopathogenic mechanisms of Acanthamoeba. In order to identify peptidases secreted by A. Nevertheless, these latter results suggest that the presence of BV-2 cells, at least at the 1: BV-2 cells were cultured in A.

Get Email Updates To receive email updates about this page, enter your email address: Below is a list of described species of Acanthamoebawith sequence types noted where known. J Electron micrograph of a BV-2 cell maintained in medium supplemented with A. The interaction of Acanthamoeba spp.

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